Basic information
Biomarker: DNA integrity index
Biomarker subtype: DNA
Clinical application: diagnosis
Histology type: endometrial carcinoma
Cohort characteristics
| Total number | Group I | Group I number | Group II | Group II number | Group III | Group III number | Group IV | Group IV number |
|---|---|---|---|---|---|---|---|---|
| 82 | EC | 60 | healthy volunteers | 22 |
Sample information
Sample type : serum
Clinical method: Quantitative PCR/Immunohistochemistry
Expression elevation: DNA integrity was calculated as the ratio of qPCR results using the 2 primer sets: qPCR-Alu247/qPCR-Alu115
Disease information
Related information
Description: Our study supports the utility of serum DNA integrity index as a noninvasive molecular biomarker in EC.
Expression figure legend: CfDNA levels related to hypertension and obesity in EC
Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791183/figure/Fig1/
Detailed Description: DNA integrity was calculated as the ratio of qPCR results using the 2 primer sets: qPCR-Alu247/qPCR-Alu115, where qPCR-Alu115 and qPCR-Alu 247 are the Alu-qPCR results obtained with the Alu115 and Alu247 primers, respectively. Because the annealing sites of Alu115 are within the Alu247 annealing sites, DNA integrity value would be 1.0 when template DNA is not truncated and 0.0 when all template DNA is truncated into fragments smaller than 247 bp. Because the Alu115 primers can amplify most fractions of circulating DNA, qPCR-Alu results obtained with Alu115 primers represent the absolute amount of DNA. The fit of the standard curve (R2) was higher than 0,99 for both Alu sequences.[PMC5791183]
