Biomarker: SerpinE2

Biomarker subtype: gene

Clinical application: prognosis(unfavorable)

Histology type: endometrial carcinoma

Country: China

Region: Guangzhou

Followed up time : 60 months

Cohort age: 36;36

Protocol age: 25;24

Control age: 11;12

Sample type : tissue

Clinical method: immunohistochemistry

Expression pattern : expression

Expression elevation: The dominant staining intensity in tumor cells was scored as 0 = negative; 1 = weak (0 ～ 25%); 2 = intermediate (26 ～ 50%); 3 = strong (51 ～ 75%); or 4 = extra strong (76 ～ 100%). We calculated the dominant staining in80 tensity from five views and added the scores together.

Validation in cell line : HTB-111 and Ishikawa cells

Validation in cell method : Cell viability assay,Cell apoptosis assay,Wound-healing assay,Migration assay,ELISA

Statictics: cutoff<55;cutoff>55

Cohort age: 36;36

Protocol age: 25;24

Control age: 11;12

Description: SerpinE2, a poor biomarker of endometrial cancer, promotes the proliferation and mobility of EC cells.

Funtion description: Knocked down SerpinE2 by siRNA in the HTB-111 and Ishikawa EC cell lines and analyzed the viability and mobility change using an MTT assay, an annexin V/PI apoptosis assay, a wound scratch test and a transwell assay. Silencing SerpinE2 induced cell apoptosis and reduced the migration ability.

Approved symbol: SERPINE2

Approved name: serpin family E member 2

Locus type: gene with protein product

HGNC ID: HGNC:8951

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:8951

Symbol status: Approved

Previous symbols: PI7

Alias symbols: pelle

Alternative gene group name: Serine (or cysteine) peptidase inhibitors

OMIM: 177010

Omim link: https://www.omim.org/entry/177010

NCBI name: 5270

NCBI link: https://www.ncbi.nlm.nih.gov/gene/5270

Summary: This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Expression: This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

NCBI gene expression https://www.ncbi.nlm.nih.gov/gene/5270#gene-expression

PDB ID: P07093

PDB link: https://www.ebi.ac.uk/pdbe/entry/search/index/?searchParams=%7B%22text%22:%5B%7B%22value%22:%22P07093%22,%22condition1%22:%22AND%22,%22condition2%22:%22Contains%22%7D%5D,%22resultState%22:%7B%22tabIndex%22:0,%22paginationIndex%22:1,%22perPage%22:%2210%22,%22sortBy%22:%22Sort%20by%22%7D%7D

BP term ID: GO:0042628|GO:0061108

BP term name: mating plug formation|seminal vesicle epithelium development

Source description: TF

Term ID: TF:M11087_1|TF:M05787

Term name: Factor: tcfec; motif: NNCATGTGACN; match class: 1|Factor: ZNF546; motif: TGGGGAACGCCAKKCGGC