Biomarker: CXCR4

Biomarker subtype: mRNA

Clinical application: treatment(theranostic target)

Histology type: endometrial carcinoma

Clinical method: RT-PCR/Western blotting

Expression pattern : expression

Validation in cell line : Ishikawa and HEC-1-A cell line

Validation in cell method : RT-PCR and Western blotting,invasion assay ,small interference RNAs of CXCR4 and CXCR7 fragments synthesized, and transfected , FCM

Description: CXCR4 is expected to become two target gene for the treatment of endometrial carcinoma.The expression levels of CXCR4 could be inhibited by RNA interference, reducing the cell proliferation, invasion in Ishikawa and HEC-1-A cells.

Funtion description: The expression levels of CXCR4 and CXCR7 could be inhibited by RNA interference, reducing the cell proliferation, invasion in Ishikawa and HEC-1-A cells. In this study, we also observed that treated with CXCR4 and CXCR7 small interfering RNA (siRNA) arrested cells in S phase. CXCL12/CXCR4 and CXCL12/CXCR7 receptor ligand systems affect the invasion of endometrial carcinoma cell line into Ishikawa and HEC-1-A. CXCR4 and CXCR7 were silenced by RNAi, which can inhibit the invasion of Ishikawa and HEC-1-A cell lines.

Approved symbol: CXCR4

Approved name: C-X-C motif chemokine receptor 4

Locus type: gene with protein product

HGNC ID: HGNC:2561

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:2561

Symbol status: Approved

Previous symbols: chemokine (C-X-C motif), receptor 4 (fusin),chemokine (C-X-C motif) receptor 4

Alias symbols: LESTR NPY3R HM89 NPYY3R D2S201E fusin HSY3RR NPYR CD184

Alternative gene group name: Chemokine (C-X-C motif) receptors | cluster of differentiation molecules", "cluster of designation molecules

OMIM: 162643

Omim link: https://www.omim.org/entry/162643

NCBI name: 7852

NCBI link: https://www.ncbi.nlm.nih.gov/gene/7852

Summary: This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Expression: Biased expression in bone marrow (RPKM 479.2), lymph node (RPKM 201.0) and 9 other tissues

NCBI gene expression https://www.ncbi.nlm.nih.gov/gene/7852#gene-expression

MF term ID: GO:0038147

MF term name: C-X-C motif chemokine 12 receptor activity

Source description: REAC | CORUM

Term ID: REAC:R-HSA-173107 |CORUM:6807 |CORUM:6808

Term name: Binding and entry of HIV virion| ADRA1A-CXCR4 complex |ADRB1-CXCR4 complex