Biomarker: CC3

Histology type: endometrial carcinoma

Country: Janpanese

Region: Kanagawa

Study type: retrospective

Followed up time :

Subgroup 1 name : low expression

Subgroup 1 number: 29

Subgroup 2 name: high expression

Subgroup 2 number: 31

Conclusion: Cleaved caspase-3 expression is a potential prognostic factor for endometrial cancer with positive peritoneal cytology.

Sample type : cytology

Sample method: immunohistochemistry

Expression pattern : Low expression

Expression elevation: Positive staining in more than 20% of tumour cells was considered ‘high’ expression (CC3High), and positive staining in less than 20% of tumour cells was considered ‘low’ expression (CC3Low).

Statictics: cutoff<50;cutoff>50

Cohort age: 14;46

Subgroup 1 age: 7;22

Subgroup 2 age: 7;24

Funtion Uniprot: Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486).

UniProt ID: P42574

UniProt Link: https://www.uniprot.org/uniprotkb/P42574/entry

Biological function from UniProt: Apoptosis

Molecular function from UniProt:

Tissue specificity from UniProt: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Subcellular UniProt: #Cytoplasm

Alternative name from UniProt:

Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position

Gene name from HGNC: CASP3 (apopain, CPP32, CPP32B, Yama)

CD antigen name: Cleaved into 2 chains:Caspase-3 subunit p17 Caspase-3 subunit p12

HPA link: https://www.proteinatlas.org/ENSG00000164305-CASP3

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Selective cytoplasmic expression in several tissues, most abundant in lymphoid tissues.

Subcellular summary HPA Located in Nucleoplasm, Mitochondria

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable) and endometrial cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000164305-CASP3/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000164305-CASP3/pathology/endometrial+cancer

HGNC ID: HGNC:1504

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1504