Biomarker: MMP9

Histology type: endometrial clear cell carcinoma

Country: UK

Study type: prospective case-control study

Followed up time :

Conclusion: A patient-friendly, urine-based test could offer a non-invasive endometrial cancer detection tool in symptomatic women. Validation in a larger independent cohort is warranted.

Sample type : urine

Sample method: mass spectrometric analysis

Funtion Uniprot: Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide

UniProt ID: P14780

UniProt Link: https://www.uniprot.org/uniprotkb/P14780/entry

Biological function from UniProt: #Collagen degradation

Molecular function from UniProt:

Tissue specificity from UniProt: Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes

Subcellular UniProt: #Extracellular matrix #Secreted

Miscellaneous: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.

Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V.

Activity regulation: Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1.

Recommended name: Matrix metalloproteinase-9

Gene name from HGNC: MMP9 (CLG4B)

HPA class: Cancer-related genes Candidate cardiovascular disease genes Disease related genes Enzymes Human disease related genes Plasma proteins Potential drug targets

AlphaFold DB: P14780

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P14780

Induction: Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. (Microbial infection) Expression induced by M.bovis MPB83 (at protein level) (PubMed:20800577).

HPA link: https://www.proteinatlas.org/ENSG00000100985-MMP9

Tissue specificity RNA from HPA: Group enriched (bone marrow, lymphoid tissue)

Tissue expression from HPA: Lymphoid tissue & Bone marrow - Innate immune response (mainly)

Single cell type specificity Group enriched (Langerhans cells, Macrophages, Hofbauer cells)

Immune cell specificity: Immune cell enriched (neutrophil)

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable), endometrial cancer (favorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000100985-MMP9/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000100985-MMP9/pathology/endometrial+cancer